Molecular characterization of biofilm in Gram-negative staphylococci for plasma coagulation testing isolated from hemodialysis patients in Diwaniyah city; A Master’s thesis.


A master’s thesis was discussed in the Department of Life Sciences, College of Education, Al-Qadisiyah University, by researcher Lamia Jabbar Abu Souda, under the supervision of Professor Dr. Bahija Abis Hamoud.
The thesis aimed to isolate and identify coagulase-negative staphylococci (CNSA) from the blood of patients and the surrounding environment using conventional and molecular diagnostic methods. The aim was also to conduct a sensitivity test on the isolated bacteria to determine their effectiveness against the isolated bacteria.
The most important conclusions were that CNSA constituted a high percentage of hemodialysis patients and the environment of the dialysis unit at Al-Dajwaniyah Teaching Hospital. S. epidermidis was also a major pathogen of bloodstream infections in hemodialysis patients. It was also largely isolated from the inpatient ward and catheterization devices, followed by S. saprophyticus.
The most important recommendations are to conduct extensive studies on the bacterial species belonging to the CNSA group and to determine their virulence factors. And their resistance to antibiotics to identify the problems and complications suffered by kidney dialysis patients and expand these studies to include more samples and sites to better understand the distribution and patterns of resistance among gram-negative staphylococci.

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