The doctoral thesis was discussed in the Department of Life Sciences, College of Science, University of Al-Qadisiyah. The doctoral thesis  by the researcher Hussam Saad Hussein Kano and under the supervision of Prof. Dr. Laith Saree Matar Al-Rikabi and Prof. Abdul-Kazem Jawad Musa.
The study aimed to evaluate the effect of different concentrations of growth regulators (NAA, 2,4-D, 2iP) in combination with jasmonic acid (JA) on stimulating callus growth and secondary metabolite production in Barhi date palms using tissue culture techniques. The study also identified the chemical compounds using a GC-MS device and evaluated their antioxidant and antibacterial activity. Hemotoxicity tests (hemolysis) were also performed, and genetic stability was verified using RAPD indices.
The results showed a significant increase in the diversity and proportions of chemical compounds at medium concentrations of jasmonic acid combined with growth regulators, along with its antibacterial activity against Staphylococcus aureus and Escherichia coli, and improved antioxidant indicators. Genetic variations occurred in tissue cultured parts.
The study recommended the use of balanced hormonal treatment (JA at medium concentrations with 2iP, 2,4-D, and NAA within experimentally established limits). In tissue culture laboratories to increase the chemical value of plant extracts, and by combining biochemical analyses with molecular markers when expanding into pharmaceutical and food applications.